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| Registros recuperados: 101 | |
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| Córdova Cabrera, Pablo Raúl. |
| Este trabajo presenta la primera exploración de plagas forestales, realizada durante el período Septiembre 2006 a Marzo 2007 en plantaciones que han sido beneficiadas por el programa de incentivos forestales PINFOR. Se hicieron recorridos terrestres a las plantaciones seleccionadas, realizando muestreos con intervalos de 15 días, en los que se identificaron árboles con síntomas anormales. y se verificó la presencia de insectos y/o árboles con enfermedades. Los insectos fueron recolectados en frascos con alcohol al 70% y en el caso de las enfermedades se confinaron partes vegetales en bolsas de plástico con condiciones de humedad. Para la identificación de los organismos se utilizaron claves taxonómicas entomológicas y fitopatológicas del... |
| Tipo: Tesis |
Palavras-chave: Plagas y enfermedades forestales; PINFOR; Identificación Forest pest and disease; PINFOR; Characterization; Identification. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1040 |
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| Martínez Hernández, Gregorio. |
| Las pitahayas son utilizadas desde tiempos prehispánicos; sin embargo, solo desde hace poco más de dos décadas se han establecido como cultivo, con gran potencial para coadyuvar al desarrollo agrícola y económico de México y otros países. Conocer la diversidad de un recurso vegetal permite elegir estrategias para su mejor aprovechamiento. Por tal motivo, el propósito de este trabajo fue la caracterización morfológica de 21 genotipos de pitahaya (Hylocereus spp.) cultivados en Tepoztlán Morelos, México. La caracterización morfológica se realizó con 28 caracteres cuantitativos y 22 cualitativos de yemas florales, flores, filocladodios y frutos. Con base en análisis de componentes principales los genotipos se diferenciaron principalmente por caracteres de... |
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Palavras-chave: Hylocereus spp.; Caracterización; Genotipos; Pitahaya; Characterization; Genotypes; Genética; Maestría. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/2131 |
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| Ramírez Jaspeado, Araceli. |
| El maíz es el cultivo más importante de México cuya superficie de siembra es de 8 millones de hectáreas con un rendimiento promedio de 3 ton/ha. Su aprovechamiento involucra los granos, tallos, espigas, raíces, olote y brácteas (“hoja para tamal”) utilizados para diversos propósitos en la alimentación humana, animal, medicinal e industrial. Se ha considerado que la principal forma de consumo del maíz es a través de la tortilla, donde, alrededor de 12.3 millones de toneladas de maíz se consumen en esta forma. En este sentido, los maíces mejorados han sido esenciales para dicho propósito, sin embargo, el uso de los maíces nativos o criollos, también ha sido de gran importancia ya que son un reservorio de la variabilidad genética y han sido la base de la... |
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Palavras-chave: Caracterización; Raza Bolita; Tlayuda; Cadena de valor; Calidad nutricional; Proteínas transgénicas; Conservación; Producción de semillas; Doctorado; Characterization; Bolita race; Value chain; Nutritional quality; Transgenic proteins; Conservation. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1822 |
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| Li,Bin; Yu,Rongrong; Liu,Baoping; Tang,Qiaomei; Zhang,Guoqing; Wang,Yanli; Xie,Guanlin; Sun,Guochang. |
| Representative strains of Serratia marcescens from an edible cactus plant and silkworms were characterized and a comparison based on their cellular fatty acid composition, 16S rRNA and groE gene sequence analysis as well as silkworm virulence and chitosan susceptibility was carried out. Results from this study indicate that there are no significant differences between the phenotypic and molecular characterization, virulence and chitosan susceptibility of the S. marcescens strains from the cactus plant and silkworms. Silkworms inoculated with S. marcescens from either plant or silkworm resulted in nearly 100% mortality. Chitosan solution exhibited strong antibacterial activity against S. marcescens. This activity increased with the increase of chitosan... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Serratia marcescens; Cactus; Silkworm; Characterization; Virulence; Chitosan susceptibility. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100013 |
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| Zarei,Mandana; Aminzadeh,Saeed; Zolgharnein,Hossein; Safahieh,Alireza; Daliri,Morteza; Noghabi,Kambiz Akbari; Ghoroghi,Ahmad; Motallebi,Abbasali. |
| Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antifungal; Characterization; Chitinase; Serratia marcescens B4A. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300022 |
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| Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques. |
| This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively.... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf |
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| Arns,C.W.; Campalans,J.; Costa,S.C.B.; Domingues,H.G.; D'Arce,R.C.F.; Almeida,R.S.; Coswig,L.T.. |
| This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and non-hemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bovine respiratory syncytial virus; BRSV; Virus isolation; Characterization; Nested RT-PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200008 |
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| Occhiuto,P.N; Peralta,I.E; Asprelli,P.D; Galmarini,C.R. |
| Paprika production is concentrated in Northwestern Argentina, where around 1500 ha are annually grown. Farmers mainly from Andean valleys, maintain their local landraces or “criollas” under different environmental conditions, using their farming traditions and selection criteria. These traditional varieties represent valuable genetic resources for conservation and selection of agronomical and quality traits. Between 2005 and 2007 eight collecting trips were made to Northwestern Argentina in order to recover local germplasm. As a result, five hundred new vegetable seed samples have been incorporated into the Germplasm Bank. Among these accessions 32 belong to Capsicum, the majority for paprika production, that were evaluated in field... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum annuum; C. baccatum; Germplasm; Characterization; Pungency; Pre-breeding. |
| Ano: 2014 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2014000200002 |
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| Lin,Ling; Kan,Xianzhao; Yan,Hao; Wang,Danni. |
| The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from... |
| Tipo: Journal article |
Palavras-chave: Bacillus thuringiensis; Cellulase; Cellulose-degrading; Characterization. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002 |
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| BARRA,BRENO; MOMM,LETO; GUERRERO,YADER; BERNUCCI,LIEDI. |
| This paper discusses the importance of studies on materials known as fillers from different mineral origins, used in asphalt mixes, specifically in the formulation of mastics. The research was carried out on samples of limestone and granite rock filler and asphalt binder (50/70). The samples were evaluated through semiquantitative chemical analyses by X-ray fluorescence, granulometry by low angle laser emission, scanning electron microscopy, softening point tests, penetration tests, and aggregate-asphalt binder and aggregate-mastic adhesion tests. The results highlighted convergent trends, indicating that the active behavior of the fillers in the mastic formulation is not related to the size of the particles, but rather to their form, surface texture,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Characterization; Fillers; Granite; Limestone; Bituminous mastics. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200995 |
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| Souza,Vanessa Ribeiro de; Silva,Ana C. G.; Pinotti,Laura Marina; Araújo,Heloísa Sobreiro Selistre; Giordano,Raquel de Lima Camargo. |
| The purpose of this work was to characterize the enzyme penicillin G acylase (PGA) produced by Bacillus megaterium. Purification of the enzyme by ultra/diafiltration did not allow the detection of the PGA band by SDS-PAGE electrophoresis due to the high content of remaining proteins. However, using the DNA of the microorganism, it was possible to replicate the genes of the two B. megaterium PGA reported in literature, showing that the enzyme consisted of two sub-units, having 245 and 537 amino acids each and an average molecular mass of 26950 and 59070 Da, respectively. The parameters studied were: 1) the influence of temperature in the 25-60(0)C range, 2) pH in the 5-10 range and 3) substrate concentration, this was tested to obtain results on the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Penicillin G acylase; Bacillus megaterium; Characterization. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400013 |
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| FACCIOLI-MARTINS, P. Y.; SOUZA, V. de; PINHEIRO, R. R.. |
| Resumo: Esta publicação tem por objetivo descrever a história, a composição da Coleção de trabalho de Microrganismos Patogênicos a Caprinos e Ovinos, e de que forma ela tem conservado, caracterizado, documentado e utilizado seus isolados. A coleção foi criada em 2012 e atualmente é composta por 141 cepas bacterianas e seis cepas virais, pertencentes às espécies Corynebacterium pseudotuberculosis, Staphylococcus aureus e ao gênero Lentivirus. Esses isolados são utilizados para produção de antígenos para imunodiagnósticos, em diagnósticos moleculares e na avaliação de resistência a antimicrobianos e ação de anti-sépticos e antimicrobianos. Participa de projetos das coleções institucionais e de gestão da qualidade. [Collection of Goat and Sheep Pathogenic... |
| Tipo: Artigo de divulgação na mídia (INFOTECA-E) |
Palavras-chave: Caracterização; Germplasm bank; Characterization; Diagnosis; Quality; Pathogenic Microorganisms; Caprino; Ovino; Banco de Germoplasma; Conservação; Resistência Genética; Diagnostico; Qualidade; Sheep diseases; Goat diseases; Germplasm conservation; Conservation banks; Genetic resistance; Small ruminants; Brazil. |
| Ano: 2020 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1123793 |
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| Wanderley,Maria Carolina de Albuquerque; Duarte Neto,José Manoel Wanderley; Lima Filho,José Luiz de; Lima,Carolina de Albuquerque; Teixeira,José António Couto; Porto,Ana Lúcia Figueiredo. |
| Abstract Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Collagenase; Fungus; Characterization; Purification; Production. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100013 |
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| OLIVEIRA,ANTÔNIA S. DE; LÓSSIO,CLÁUDIA F.; RANGEL,ANNE J.; MARTINS,MARIA G.Q.; NASCIMENTO,FERNANDO E.P. DO; ANDRADE,MARIA L.L. DE; CAVADA,BENILDO S.; LACERDA,SÍRLEIS R.; NASCIMENTO,KYRIA S. DO. |
| ABSTRACT Freshwater algae are rich sources of structurally biologically active metabolites, such as fatty acids, steroids, carotenoids and polysaccharides. Among these metabolites, lectins stand out. Lectins are proteins or glycoproteins of non-immune origin which bind to carbohydrates or glycoconjugates, without changing ligand structure. Many studies have reported on the use of Spirogyra spp. as effective bioindicators of heavy metals; however, reports on Spirogyra molecular bioprospecting are quite limited. Therefore, this study aimed to detect, isolate, purify and characterize a lectin present in the freshwater green algae Spirogyra. Presence of the lectin protein in the extract was detected by hemagglutination assays. Subsequently, the protein extract... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Algae; Characterization; Lectin; Purification. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502113 |
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| Dhillon,Pallavi; Mirza,Mohd. Aamir; Anwer,Md. Khalid; Alshetaili,Abdullah Saud; Alshahrani,Saad Maria; Iqbal,Zeenat. |
| The foremost aim of the current research was to prolong and sustain the release of erythromycin (ERY) by preparing a solid lipid nanoparticles (SLNs)-based gel formulation for the safe and effective treatment of acne. ERY-loaded SLNs were developed, and various process variables were optimized with respect to particle size, zeta potential, and entrapment efficiency using the Taguchi model. The average particle size, PDI, zeta potential, drug entrapment efficiency, and drug loading of optimized SLN (F4) were found to be 176.2±1.82 nm, 0.275±0.011, -34.0±0.84, 73.56%, and 69.74% respectively. The optimized SLN (F4) was successfully incorporated into the carbopol-based hydrogel. The in vitro release of ERY from the SLN gel and plain gel were compared and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Erythromycin; Solid lipid nanoparticles; Nanogel; Taguchi model; Characterization; Diffusion disc. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100583 |
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| Registros recuperados: 101 | |
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